Aug 17, 2014 itaconic acid is on the doe department of energy top 12 list of biotechnologically produced building block chemicals and is produced commercially by aspergillus terreus. To understand the regulation of itaconic acid production, bonnarme et al. Efficient itaconic acid production from glycerol with. Name date 11 csource fermentation pilot c6 cellulose labscale c6 cellulose labscale glucose end of fermentation h 115 146 260 itaconic acid produced gl 65. Alongside the production of drugs and flavors as well as amino acids, biobased monomers and polymers and biofuels, basic insights are also given as to. Pdf itaconic acid a biotechnological process in change. Itaconic acid ia production by aspergillus terreus mjl05 strain was investigated in submerged batch fermentation in a stirred bioreactor to determine the effect of varying the nitrogen, phosphorous and carbon sources concentrations in the production medium. Manganese deficiency is required for high itaconic acid. Itaconic acid is an unsaturated dicarbonic acid which has a high potential as a biochemical building block, because it can be used as a monomer for the production of a plethora of products including resins, plastics, paints, and synthetic fibers. These acids are used as building blocks for large numbers of compounds, such as resins, paints, plastics, and synthetic fibers acrylic plastic, super absorbants, and antiscaling agents 67.
Pdf synthesis of itaconic acid from agricultural waste. The initial and previously tested conditions for corn cob fermentation section 2. Pdf solidstate fermentation technology for bioconversion of. And also extraction of itaconic acid from the fermentation was performed with 91. Physical and chemical properties physical state powder solid appearance offwhite odor strong odor threshold no information available ph 3. Itaconic acid is used as a biobased, renewable building block in the polymer industry. The cac intermediate cisaconitate is enzymatically processed by cisaconitate dehycarboxylase cada to produce itaconic acid 68. The aspergillus terreus ncim657 was selected for the production of itaconic acid in submerged fermentation, the aspergillus terreus ncim657 produced itaconic acid over the entire range of ph 1. Mass balance of the fermentation with 110 g l1dxylose revealed that. Itaconic acid market size potential exceeds usd 290 million by 2024. Itaconic acid is on the doe department of energy top 12 list of biotechnologically produced building block chemicals and is produced commercially by aspergillus terreus. However, the production cost of itaconic acid is too high to be economically competitive with the petrochemicalbased products. Glycerol, a biodiesel byproduct was reported as an efficient substrate to achieve high itaconic acid productivities. Aug 27, 2012 aspergillus niger was selected as a host for producing itaconic acid due to its versatile and tolerant character in various growth environments, and its extremely high capacity of accumulating the precursor of itaconic acid.
The biosynthetic pathway of itaconic acid is like citrate biosynthesis, where. Sugarcane pressmud or peeled sugarcane pressmud is the support used to adsorb liquid medium for the production of itaconic acid by an aspergillus terreus mutant strain. Although some citric acid is produced by solid state fermentation, the estimated 2. This volume of the series is on solidstate fermentation. Virtually all itaconic acid produced is by fermentation by specific strains of a. It is produced by submerged fermentations of the filamentous fungus. Itaconic acid market size, share, price report 2024. Expressing the cad gene from aspergillus terreus opened the metabolic pathway towards itaconic acid in a. The method to produce itaconic acid is a solidstate fermentation method.
Asia pacific dominated the global itaconic acid market both in terms of volume and revenue in 2014. Furthermore, considering that citric acid is a key precursor of the itaconic acid biosynthesis, citrate synthase cs, which is encoded by cita and catalyses an irreversible reaction, may play an important role in improving itaconic acid production. Production of itaconic acid by ustlago maydis from agro wastes in solid state fermentation. Sigmaaldrich offers a number of itaconic acid products. The transformants of pxh59 and pxh61were tested for itaconic acid production using saccharified corn starch hydrolysates 140 gl glucose equivalent as the starting material on a rotary shaker at 37c for 72 h.
Exploring saccharomyces cerevisiae to improve microbe. Pdf itaconic acid ia is one of the hopeful substances within the cluster of organic acids. Efficient recovery of ia from aqueous fermentation broth was previously achieved by aminebased reactive extraction re systems. Aspergillus niger was selected as a host for producing itaconic acid due to its versatile and tolerant character in various growth environments, and its extremely high capacity of accumulating the precursor of itaconic acid. Metabolic engineering strategies to improve yeast based. In the present work, several back extraction methods were tested. Pdf microbial production of itaconic acid researchgate. Citric acid production on three cellulosic supports in. These metrics are regularly updated to reflect usage leading up to the last few days. Itaconic acid, or methylidenesuccinic acid, is an organic compound. Both acids are excellent platform chemicals with a wide range of applications in different market, such as plastics, coating, or. Organic acids represent the thirdlargest category among other products. Historically, itaconic acid was obtained by the distillation of citric acid, but currently it is produced by fermentation. For increasing the productivity, the influence of starting substrate concentration, the optimum timing of.
Itaconic acid also called methylene succinic acid is a white crystalline carboxylic acid obtained by the fermentation of carbohydrates. Feb 14, 20 itaconic acid is an unsaturated dicarbonic acid which has a high potential as a biochemical building block, because it can be used as a monomer for the production of a plethora of products including resins, plastics, paints, and synthetic fibers. Current advances in solid state fermentation provides knowledge and information on solid state fermentation involving the basics of microbiology, biochemistry, molecular biology, genetics and principles of genetic engineering, metabolic engineering and biochemical engineering. Citric acid production by solidstate fermentation on a semipilot. Fermentatative production of itaconic acid by aspergillus.
Itaconic acid is exclusively produced this way bafana and pandey 2018. Itaconic acid is commercially produced by the cultivation of aspergillus terreus with molasses. In this study, the yields of itaconic acid and fumaric acid line 28 were not higher than that of previous study line 85. A solid state fermentation method was developed for the production of citric acid from date pulp obtained of. Us5336744a process for polymerization of itaconic acid.
Production of citric acid from date pulp by solid state fermentation. Pdf production of itaconic acid by ustlago maydis from. However, maximum itaconic acid production was observed at ph 2. Solidstate fermentation for the production of organic. Among them, itaconic acid is an important organic acid. This dicarboxylic acid is a white solid that is soluble in water, ethanol, and acetone. Microbial production of citric acid by solid state fermentation of kiwi fruit peel. Exploring saccharomyces cerevisiae to improve microbebased production of itaconic acid nicole martins rodrigues thesis to obtain the master of science degree in biotechnology supervisor. Recently, several ustilaginaceae were reported to produce organic acids from glycerol, which is the main side stream in biodiesel production. Moreover, we can find that the yield of solid fermentation was much lower than that of liquid fermentation, so the benefits of itaconic acid and fumaric acid production by solid fermentation need to be further evaluated. Fermentation process for the production of itaconic acid was carried out using jatropha seed cake. The latest volume in the advanced biotechnology series provides an overview of the main product classes and platform chemicals produced by biotechnological processes today, with applications in the food, healthcare and fine chemical industries.
In this study, we present ustilago vetiveriae as new production organism for itaconate synthesis from glycerol. Pdf in the last years, itaconic acid has gained increasing interest as future biobased platform chemical. Improving itaconic acid production through genetic. Rising demand for biobased chemical intermediates for sustainable products will drive itaconic acid market growth, which offers an ideal platform. Efficient itaconic acid production by aspergillus terreus overcoming the strong inhibitory effect of manganese. Separation of itaconic acid from the fermented broth has a considerable impact in the total production cost. Current developments in biotechnology and bioengineering. One of the most important uses of this biomonomer is the environmentally sustainable production of biopolymers. The bioproduction of highvalue chemicals such as itaconic and fumaric acids ia and fa, respectively from renewable resources via solidstate fermentation ssf represents an alternative to the current bioprocesses of submerged fermentation using refined sugars. Direct production of itaconic acid from liquefied corn. As there is no indigenous commercial production of itaconic acid, hitherto, in india, the present study. Enhanced itaconic acid production in aspergillus niger. The genetic engineering approach has been extensively.
Itaconic acid, used as a comonomer at a level of 15% for certain polymer products, is an important constituent in the fabrication of synthetic fibres, coatings, adhesives, thickeners and binders. Unsaturated solid bond makes a conjugated system with carbonly group. Biobased itaconic acid is expected to find its application in manufacturing adhesives and sealants and is expected to foresee growth on account of development of biobased adhesives and inclination of chemical manufacturers towards. Article views are the countercompliant sum of full text article downloads since november 2008 both pdf and html across all institutions and individuals. News itaconic acid and methacrylic acid as chemical building blocks of the future published on may 26, 2016. Pfizer obtains volume output of itaconic acid by fermentation process. Oct 10, 1995 itaconic acid andor salt thereof is produced via aerobic microbial fermentation, for example by means of the species aspergillus terreus or aspergillus itaconicus, of a nutrient medium containing a source of assimilable carbon, such carbon source at least in part comprising an effective amount of glycerol. Itaconic acid andor salt thereof is produced via aerobic microbial fermentation, for example by means of the species aspergillus terreus or aspergillus itaconicus, of a nutrient medium containing a source of assimilable carbon, such carbon source at least in part comprising an effective amount of glycerol. Itaconic acid is an example of a dicarbonic unsaturated acid.
Then you can start reading kindle books on your smartphone, tablet, or computer. The authors showed that during itaconic acid synthesis, the concentrations of pyruvic acid, oxaloacetic acid, and citric acid were increased. Nov 25, 2016 he has authored more than 150 scientific publications, more than 20 books and book chapters, holds more than 20 patents and has received several scientific awards, including the young scientist award of the european federation of biotechnology, and is board member of various scientific journals. Itaconic acid production is a further perversion of the krebs cycle, citrate is converted as normally into cisaconitate, which for reasons unknown is, in some organisms, decarboxylated into itaconitate, which has no known metabolic role in the cell. Effect of different cultural conditions on production of. Itaconic acid is a promising chemical that has a wide range of applications and can be obtained in large scale using fermentation processes. This study focused on the production of itaconic acid from a lignocellulosebased carbon source by a. Itaconic acid definition of itaconic acid by the free.
Pdf biochemistry of microbial itaconic acid production. The production of several useful organic components such as lactic acid, gluconic acid, itaconic acid and several other organic acids using microorganisms has been practiced since many years as microbes are a rich source of these components. Polymers of itaconic acid are formed at high conversion by an aqueous polymerization process of partially neutralized monomer solution, water, polyvalent metal ion and initiator. Novel intensified back extraction process for itaconic. Itaconic acid ia, an unsaturated dicarboxylic acid produced by fermentation, is a promising alternative to petrochemicalbased monomers as a building block for plastics, resins, and synthetic fibers. Itaconic acid and methacrylic acid as chemical building. Optimized bioproduction of itaconic and fumaric acids.
Citations are the number of other articles citing this article, calculated by crossref and updated daily. Study of itaconic acid production by aspergillus terrus. Itaconic acid is a potential green chemical to substitute maleic anhydride in upr production owing to its similar structure and has potential to be a good substitute. The production of several useful organic components such. The name itaconic acid was devised as an anagram of aconitic acid, another derivative of citric acid. Downstream process development in biotechnological. Jatropha seed cake is one of the best carbon sources among various carbohydrates, because it is pure, inexpensive and available in a mass supply. Enter your mobile number or email address below and well send you a link to download the free kindle app.
Pdf itaconic acid is an unsaturated dicarbonic acid which has a high. Commercially production of citric acid is generally by submerged fermentation of sucrose or molasses using the filamentous fungus aspergillus niger rohr et al. Cassava bagasse was found to be a better substrate than vegetable sponge and sugar cane bagasse. Improved itaconic acid production from undetoxified. Nov 15, 2016 itaconic acid is a promising chemical that has a wide range of applications and can be obtained in large scale using fermentation processes. Solidstate fermentation for the production of organic acids. Pdf fungal fermentation of lignocellulosic biomass for. High concentration of production capacities due to the shutdown of the manufacturing plants of itaconic acid in other parts of the world has led to this growth. Production of organic acids from agroindustrial residues.
About frontiers institutional membership books news frontiers. Itaconic acid is generally produced from raw corn starch, including three steps. Most of the chapters in this book include information on the variety of agricultural. The bioproduction of highvalue chemicals such as itaconic and fumaric acids ia and fa, respectively from renewable resources via solidstate fermentation. Historical evolution of itaconic acid production, based on a search at scopus. Optimized bioproduction of itaconic and fumaric acids based. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Itaconic acid a biotechnological process in change. Development of solid state fermentation is expected to open new avenues for biobased itaconic acid market. Itaconic acid is an organic acid that is used as a platform chemical for the production of various value added chemicals such as polyitaconic acid, resins biofuel components, ionomer cements etc. Polymers produced according to the process of the invention are useful as detergent additives, scale inhibitors and removers, sequestrants, yarn sizers, deflocculating agents, deinking agents, suspending agents and. China itaconic acid market size is projected to surpass usd 20 million valuation by 2024. The authors showed that during itaconic acid synthesis, the concentrations. He has authored more than 150 scientific publications, more than 20 books and book chapters, holds more than 20 patents and has received several scientific awards, including the young scientist award of the european federation of biotechnology, and is.